Nhap mon cong nghe sinh hoc

Công nghệ sinh học trong sản xuất kit chẩn đoán, thuốc điều trị bệnh và thức ăn cho vật nuôi
Công nghệ sản xuất kháng thể đơn dòng

Vacxin tái tổ hợp

Các sinh phẩm kích thích sinh trưởng vật nuôi

MAS trong ứng dụng chẩn đóan bệnh vật nuôi
Vaccine
Recombinant vaccine
Conventional vaccine
Inactive
Weak
Gene transfer
Virus protein (Antigen)

FMDV
Marek
Gumboro
Newcastle
Transfer gene coated glycoprotein of Newcastle virus into smallpox
Transfer gene hemagglutini of influenza into pox virus
DNA vacine
Injection of pure DNA/RNA preparation to muscle
Uses of vector (vaccinia, E. coli., adenovirus, retroviruses..) for delivery gene
Reimplantation of autologous cells (cell of individual to be vaccinated)
in to which the gene has been transferd
Particle gun delivery of plasmid DNA which contain the gene
in an expression cassete
Disease diagnosis
1. Probes
DNA/RNA- PCR
2. Monoclonal antibodies
Ag isolation
injection
Spleen cells isolation
Marrow cancer cell (Myeloma )
Cell fusion
Hybridized cells selected in HAT
(hypoxathin aminopterin, Thymidine )
Cell culture (separated)
Antibody in medium
Y
Y
Y
Checking hybridized cells in medium
with MAb (reaction with Ag)
MAb Isolated from medium
Steps for MAb production
Disease diagnosis
3. Detect genetic disease
4. Disease treatment
5. Product from nonrecombinant organisms
(Micro-organisms, Plant and animail cell culture)
6. Product from recombinant organisms
(Genetically engineered Micro-Organism
Animal cell culture, transgenic plants)
Hormone tăng trưởng gà
Hormone tăng trưởng heo
(hGH, bGH,
Hormone tăng trưởng Cá
(hGH, fGH, bGH, delta-crystalline gà
, beta-galactosidase vi khuẩn, AFP, …
Hormone tăng trưởng cừu
(hGH, bGH, sGH…mMT…)
Hormone tăng trưởng bò
( bGH)
Probes
DNA/RNA- PCR
DHF (Dengue higher fever)

Hepatic B, C (HBV, HCV)

WSSV-White spot syndrom virus)